I've had a subscription to Chat GPT4.0 since February, and they've just added the ability to upload pictures that it can read and discuss.
Here are five examples.
The image depicts a laboratory setting with test tubes filled with colorful liquids, equipment in the background, and a chest or box in the foreground that's secured with chains and a padlock. The imagery could suggest the importance of protecting or securing something valuable or confidential in a scientific context. How can I assist you further regarding this image?
It appears to be a table detailing various medical tests, their codes, descriptions, and cost variations between May 2023 and October 2023. How can I assist you with this information?
What is the average price in the May 2023 column?
To calculate the average price for May 2023, I'll sum up the prices listed in the May 2023 column and then divide by the number of entries.
Prices for May 2023 are:
$1,264, $3,084, $1,374, $3,500, $2,920, $1,264
Sum = $1,264 + $3,084 + $1,374 + $3,500 + $2,920 + $1,264 = $13,406
Number of entries = 6
Average price for May 2023 = $13,406 ÷ 6 = $2,234.33
The average price in the May 2023 column is $2,234.33.
The image appears to show results from a western blot experiment, a technique used in molecular biology to detect proteins in a sample.
Panel A is labeled "FAIL" and displays multiple bands on the blot when probed with the "Mouse anti CA9" antibody, indicating non-specific binding or other issues. The desired band, which is likely represented by the arrow labeled "CA9," is amongst several other undesired bands.
Panel B is labeled "PASS" and shows a clean and specific band for the sample when probed with the "Mouse anti CDK2" antibody, as indicated by the arrow labeled "CDK2". This suggests that the antibody specifically recognized its target protein without non-specific bindings.
In a research or diagnostic context, the clarity and specificity of bands, as seen in Panel B, are desirable because they provide confidence in the results. On the other hand, the multiple bands in Panel A can introduce uncertainty about which band represents the target protein and which are artifacts or non-specific bindings.
In Panel A, which of the columns shows the largest or heaviest blot?
In Panel A, the column showing the largest or heaviest blot is the one just to the right of the "CA9" arrow label. This blot is darker and more prominent compared to the other visible bands in the same panel.
[does he mean to the left?]
I read the column header as MCF-7, but it is written at an angle. Can you confirm or agree?
Based on the image resolution provided and the content visible to me, I can't definitively identify the exact text of the column header above the largest or heaviest blot. If you read it as "MCF-7", I'll trust your observation since you have the direct visual, but I cannot confirm it based on the image alone. If you need further clarification or analysis, I'm here to help!
This image provides a clear and concise representation of the workflow for western blotting, a common technique used in molecular biology to detect and quantify specific proteins in a sample. Here's a step-by-step breakdown based on the provided graphic:
Gel Electrophoresis: Cellular proteins are taken and coated with a compound called SDS (sodium dodecyl sulfate) to give them a negative charge. These proteins are then loaded onto a gel and separated based on their size by applying an electric current. Smaller proteins move faster through the gel, while larger proteins move slower.
Transfer (Blot): The separated proteins on the gel are then transferred or blotted onto a membrane. This process ensures that the protein pattern from the gel is now on a more stable membrane, usually made of nitrocellulose or PVDF.
Block: To prevent non-specific binding of antibodies in the subsequent steps, the membrane is coated or blocked with a generic protein. This usually involves incubating the membrane in a solution containing milk proteins or bovine serum albumin (BSA).
Wash: Excess blocking agent is washed away to prepare for the antibody incubation.
Primary Antibody Binding: The membrane is incubated with a primary antibody that specifically recognizes and binds to the target protein.
Wash: Any unbound primary antibody is washed away.
Secondary Antibody Binding: A secondary antibody is added, which binds to the primary antibody. This secondary antibody often has a detectable label, like an enzyme or a fluorescent dye, which allows for visualization in the next step.
Wash: Any unbound secondary antibody is washed away.
Visualize: The presence and amount of the specific protein are then visualized, often using a chemiluminescent reaction, fluorescence, or colorimetric detection.
The workflow ends with bands on the membrane, where the intensity and position of each band correspond to the amount and size of the target protein, respectively.
This visual representation provides a great overview of the western blotting process. How can I assist you further regarding this?
The image illustrates a simplified representation of a Western Blot Analysis.
On the left side of the image, there are three rows representing bands from a Western Blot experiment. These bands correspond to the protein levels of:
Phospho ERK: This indicates the phosphorylated form of the ERK protein. Phosphorylation is a common post-translational modification in proteins that can alter their activity.
ERK: This row shows the total ERK protein levels, regardless of its phosphorylation status.
B-Actin: [Corrects the spelling from 'action' (sic)] Often used as a loading control in Western Blot experiments, B-Actin levels help ensure that roughly equal amounts of protein samples were loaded in each well.
The columns labeled "Ctr 1, Ctr 2, Ctr 3" presumably represent control samples, while those labeled "Trt 1, Trt 2, Trt 3" likely stand for treated samples. By comparing band intensities between the control and treated samples, researchers can infer changes in protein levels or activity due to the treatment.
On the right side, there's an illustration of a pipette adding a sample to a test tube and a representation of a gel electrophoresis setup. This is the initial step of the Western Blot process, where proteins are separated based on their size in an electric field.
From the displayed bands, one could make various inferences about the effect of the treatment on ERK and its phosphorylation levels, especially when compared to the control.
This picture is a selfie of a man wearing glasses, a blue cap with a logo on it, and a grayish sweater. He's standing in front of a graffiti-covered wall. Prominently, there's an artwork of a character that resembles Snoopy from the "Peanuts" comic strip, rendered with silver and yellow colors and some abstract patterns. The artwork has been tagged and overlaid with other graffiti elements, including a padlock that's attached to the surface, further adding to the urban and street-art feel of the image. The man is capturing just half of his face in the frame, emphasizing more on the street art behind him.
This is a picture of a meal served on an outdoor table. On the plate, there are several items:
- Toasted bread, which seems to be dusted with some seasoning or possibly powdered sugar, accompanied by a syrup or sauce in the center.
- An omelette or scrambled eggs topped with slices of bacon.
- A serving of hash browns or shredded potatoes.
- Two small containers, one with a greenish sauce (possibly salsa verde) and the other with a red sauce (possibly ketchup or hot sauce).
To the right of the plate, there's a black and white fluffy dog wearing a pink harness, looking attentively. The dog is tethered with a pink and white polka-dotted leash. The setting appears to be a sunny outdoor patio or cafe, given the presence of the metal mesh table and the sunlight. The person taking the picture is seated, as indicated by the glimpse of their leg and shoe at the bottom right of the image.